Gum acacia-PVA hydrogel blends for wound healing

, , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00009-4
First Page: 78
Last Page: 91
Views: 587


Keywords: Wound healing, Gum acacia, Hydrogels, Oxygen permeability, Biodegradability, Hemocompatibility


Abstract


Hydroactive wound dressings ensure a physiologically moist wound milieu which enhances healing and patient comfort. Polymers like polyurethane, salts of alginic acid, collagen, cellulose and other gelable polysaccharides are commonly used for preparation of hydrogels but have their own limitations. In the present study acacia gum, a natural polysaccharide having known antioxidant and wound healing properties has been blended with polyvinyl alcohol and prepared hydrogel matrix further explored for its wound healing potential. Structural characterization of blended films by FTIR and XRD method showed that polysaccharide gum associated with PVA molecules via acetal bridges and they were amorphous in nature. The gel exhibited free swell capacity of 64 g 0.100 cm−2, which is comparable to the commercially available films recommended for heavily exuding wounds. The GA/PVA blends showed 34% moisture retention (Rh) ability after 24 h and their fluid absorbing (26%) and fluid donation (16%) ability make them suitable for moist and fibrinous wounds. They also exhibited blood compatibility, oxygen permeability, bacterial impermeability, antioxidant activity, iron chelation ability and biodegradability. In vivo healing potential has been evaluated on Swiss albino mice where hydrogel coated wounds showed faster and scar-less wound regeneration. In vitro Povidone-Iodine loading and release studies showed that iodine release followed Fickian diffusion process. Prepared hydrogels are breathable and hydrophilic in nature and are able to maintain appropriate moisture level at wound surface, suitable for acceleration of wound healing process. Based upon the studied properties the designed hydrogels are recommended for dry, necrotic and low exuding wounds.

Wound healing, Gum acacia, Hydrogels, Oxygen permeability, Biodegradability, Hemocompatibility


*Pdf Download Buy Printed Copy

(*Only SPR Members can download pdf file; #Open Access;)

References


  1. Abu-Dalo MA, Othman AA, Al-Rawashdeh NAF (2012) Exudate gum from Acacia trees as green corrosion inhibitor for mild steel in acidic media. Int J Electrochem Sci 7:9303–9324

  2. Ahmed Mohamed H, Mohamed Al-Shaigi RS (2014) The impact of marketing strategy on export performance (case study of Sudan gum arabic export performance). Int J Environ Sci Tech 3:1618–1635

  3. Albert M (2008) The role of hyperbaric oxygen therapy in wound healing. Wound Care Canada 6:60–62

  4. Ali BH, Al Moundhri MS (2006) Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food Chem Toxicol 44:1173–1183

  5. Ali AA, Ali KE, Fadlalla A, Khalid KE (2008) The effects of GA oral treatment on the metabolic profile of chronic renal failure patients under regular haemodialysis in Central Sudan. Nat Prod Res 22:12–21

  6. American Society for Testing and Materials ASTM E (1996) Standard test methods for water vapour transmission of materials ASTM E 96–93. American Society for Testing and Materials, Philadelphia

  7. American Society for Testing and Materials ASTM F (2000) Standard practices for assessment of haemolytic properties of materials. American Society for Testing and Materials, Philadelphia

  8. Angel DE, Morey P, Storer JG, Mwipatayi BP (2008) The great debate over iodine in wound care continues: a review of the literature. Wound Pract Res 6:6–21

  9. Balakrishnan B, Mohanty M, Umashankar PR, Jayakrishnan A (2005) Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin. Biomaterials 26:6335–6342

  10. Bhatnagar M, Parwani L, SharmaV Ganguli J, Bhatnagar A (2013) Hemostatic, antibacterial biopolymers from Acacia arabica (Lam.) Willd. and Moringa oleifera (Lam.) as potential wound dressing materials. Indian J Exper Biol 5:804–810

  11. Boateng JS, Matthews KH, Stevens HNE, Eccleston GM (2008) Wound healing dressings and drug delivery systems: a review. J Pharm Sci 97:2892–2923

  12.  

  13. Bruin P, Jonkman MF, Meijer HJ, Permings AJ (1990) A new porous polyether urethane wound covering. J Biome Mater Res 24:217–226

  14. Chakavala SR, Patel NG, Pate NV, Thakkar VT, Patel KV, Gandhi TR (2012) Development and in vivo evaluation of silver sulfadiazine loaded hydrogel consisting polyvinyl alcohol and chitosan for severe burns. J Pharm Bioallied Sci 4:S54–S56

  15. Chime Salome A, Onunkwo Godswill C, Onyishi Ikechukwu I (2013) Kinetics and mechanisms of drug release from swellable and non swellable matrices: a review. Res J Pharm Biol Chem Sci 4:97–103

  16. Dinis TCP, Madeira VMC, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophy 315:161–169

  17. Edwards R, Harding KG (2004) Bacteria and wound healing. Curr Opin Infect Dis 17:91–96

  18. Eming SA et al (2006) A novel property of povidon-iodine: Inhibition of excessive protease levels in chronic non-healing wounds. J Invest Dermatol 126:2731–2733

  19. Esa NM, Hern FS, Ismail A, Yee CL (2010) Antioxidant activity in different parts of roselle (Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds. Food Chem 122:1055–1060

  20. Field FK, Kerstein MD (1994) Overview of wound healing in a moist environment. Am J Surg 167:2S–6S

  21. Flory PJ (1953) Principles of polymer chemistry. Cornell University Press, Ithaca

  22. Giacometti A, Cirioni O, Greganti G, Fineo A et al (2002) Antiseptic compounds still active against bacterial strains isolated from surgical wound infections despite increasing antibiotic resistance. Eur J Clin Microbiol Infect Dis 21:553–556

  23. Glover DA, Ushida K, Phillips AO, Riley SG (2009) Acacia (sen) Supergum (TM) (Gum arabic): an evaluation of potential health benefits in human subjects. Food Hydrocolloid 23:410–415

  24. Gulrez S, Al-Assaf S, Phillips GO (2011) Hydrogels: methods of preparation, characterization and applications. In: Capri A (ed) Progress in molecular and environmental bioengineering from analysis and modelling to technology applications. InTech, Rijeka, Croatia, pp 117–150

  25. Halliwell B (1989) Protection against tissue damage in vivo by desferrioxamine: What is its mechanism of action? Free Radical Bio Med 7:645–651

  26. Hampton S (2011) KerraMax®: managing highly exuding wounds. J Community Nurs 25:4–8

  27. Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Deliv Rev 54:3–12

  28. Huang X, Brazel CS (2001) On the importance and mechanisms of burst release in matrix-controlled drug delivery systems. J Control Release 73:121–136

  29. Ibrahim MS, Ibrahim SM, Farag SA (2007) Characterization, thermal and mechanical behaviours of gamma irradiated gum Arabic/polyvinyl alcohol polymer blends. Poly Plast Tech Eng 46:1143–1149

  30. Imai Y, Nose YJ (1972) New method for evaluation of antithrombogenicity of materials. J Biomed Mater Res 6:165–172

  31. International Organization for Standardization ISO 10993–10 (2003) Biological evaluation of medical devices-Part 10: tests for irritation and delayed-type hypersensitivity

  32. Islam AM, Phillips GO, Sljivo A, Snowden MJ, Williams PA (1997) A review of recent developments on the regulatory, structural and functional aspects of gum arabic. Food Hydrocolloid 11:493–505

  33. Jahan N, Afaque SH, Khan NA, Ahmad G, Ansari AA (2008) Physico-chemical studies of gum acacia. Nat Prod Rad 7:335–337

  34. Jary A, Lurton Y, Gicquel V, Abault Y, Basle B (2007) Evaluation of the fluid affinities of amorphous hydrogel dressings. Le Journal des Plaies et Cicatrisations 58:97–100

  35. Jayaraja Kumar K, Hemanth Kumar Reddy C, Gunashakaran V, Ramesh Y (2009) Application of broad spectrum antiseptic povidone iodine as powerful action: a review. J Pharma Sci Technol 1:48–58

  36. Kaith BS, Kumar K (2007) Preparation of Psyllium mucilage and acrylic acid based hydrogels and their application in selective absorption of water from different oil/water imulsions. Iran Polym J 16:529–538

  37. Katayama T, Nakauma M, Todoriki S, Phillips GO, Tada M (2006) Radiation-induced polymerization of gum arabic (Acacia sengal) in aqueous solution. Food Hydrocolloid 20:983–989

  38. Kumari A, Kaith BS, Singh AS, Kalia S (2010) Synthesis, characterization and salt resistance swelling behavior of psy-g-poly(AA) hydrogel. Adv Mater Lett 1:123–128

  39. Lay-Flurrie K (2004) The properties of hydrogel dressings and their impact on wound healing. Prof Nurs 19:269–273

  40. Leitão AF, Gupta S, Silva JP, Reviakine I, Gama M (2013) Hemocompatibility study of a bacterial cellulose/polyvinyl alcohol nanocomposite. Colloid Surface B 111:493–502

  41. Litwin CM, Rayback TW, Skinner J (1996) Role of catechol siderophore synthesis in Vibrio vulnificus virulence. Infect. Immun 64:2834–2838

  42. Liu K, Li Y, Xu F, Zuo Y, Zhang L, Wang H, Liao J (2009) Graphite/poly (vinyl alcohol) hydrogel composite as porous ringy skirt for artificial cornea. Mater Sci Eng C 21:261–266

  43. Lu G, Ling K, Zhao P et al (2010) A novel in situ-formed hydrogel wound dressing by the photocross-linking of a chitosan derivative. Wound Repair Regen 18:70–79

  44. Mansur HS, Sadahira CM, Souza AN, Mansur AAP (2008) FTIR spectroscopy characterization of poly (vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde. Mater Sci Eng C 28:539–548

  45. Maslin BR, Miller JT, Seigler DS (2003) Overview of the generic status of Acacia (Leguminosae: Mimosoideae). Aus Syst Bot 16:1–18

  46. Mishra A, Chaudhary N (2010) Study of povidone iodine loaded hydrogels as wound dressing material. Trends Biomater Artif Organs 23:122–128

  47. Nwomeh BC, Liang HX, Diegelmann RF, Cohen IK, Yager DR (1998) Dynamics of the matrix metalloproteinases MMP-1 and MMP-8 in acute open human dermal wounds. Wound Repair Regen 6:127–134

  48. Oyaizu M (1986) Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–315

  49. Parwani L, Bhatnagar M, Bhatnagar A, Sharma V (2012) Reactive oxygen species control by plant biopolymers intended to be used in wound dressings. IJPPS 4:506–510

  50. Qiu Y, Park K (2001) Environment-sensitive hydrogels for drug delivery. Adv Drug Del Rev 53:321–339

  51. Rehman KU, Wingertzahn MA, Teichberg S, Harper RG, Wapnir RA (2003) Gum arabic. (GA) modifies paracellular water and electrolyte transport in the small intestine. Dig Dis Sci 48:755–760

  52. Rezanejade Bardajeea G, Pourjavadi A, Soleymanb R, Sheikh N (2010) Gamma irradiation mediated synthesis of a new superabsorbent hydrogel network based on poly (acrylic acid) grafted onto salep. J Iran Chem Soc 7:652–662

  53. Sadeghi M, Hosseinzadeh H (2008) Synthesis and swelling behavior of starch-poly (sodium acrylate-co-acrylamide) superabsorbent hydrogel. Turk J Chem 32:375–388

  54. Sadeghi M, Hosseinzadeh H (2011) Synthesis and properties of biopolymer based on gelatin-G-poly (sodium acrylate-co-acrylamide) for cephalexin controlled release. Turk J Biochem 36:334–341

  55. Sadeghi M, Ghasemi N, Kazemi M, Soleimani F (2012) Synthesis, swelling behavior, salt and pH sensitivity of cross linked carrageenan-graft-poly (acrylamide-co-itaconic acid) superabsorbent hydrogel. Middle-East J Sci Res 11:311–317

  56. Sedlarik V, Saha N, Sedlarikova J, Saha P (2008) Biodegradation of blown films based on poly (lactic acid) under natural conditions. Macromol Symp 272:100–103

  57. Segal L, Creely JJ, Martin AE Jr, Conrad CM (1962) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794

  58. Simon DA, Dix FP, McCollum CN (2004) Management of venous leg ulcers. Br Med J 328:1358–1362

  59. Singh B, Sharma S, Dhiman A (2013) Design of antibiotic containing hydrogel wound dressings: biomedical properties and histological study of wound healing. Int J Pharm 45:782–791

  60. Soler DM, Rodriguez Y, Correa H, Moreno A, Carrizales L (2012) Pilot scale-up and shelf stability of hydrogel wound dressings obtained by gamma radiation. Radiat Phys Chem 81:1249–1253

  61. Street C, Anderson DMW (1983) Refinement of structures previously proposed for gum arabic and other acacia gum exudates. Talanta 30:887–893

  62. Surgical Materials Testing Laboratory SMTL TM 238 (2005) Test method for fluid affinity of hydrogels. Report, Wales

  63. Tarun K, Gobi N (2011) Calcium alginate/PVA blended nano fibre matrix for wound dressing. Ind J Fibre Textile Res 37:127–132

  64. Thomas S, Hay NP (1994) Assessing the hydroaffinity of hydrogel dressings. J Wound Care 3:89–92

  65. Thomas S, Hay NP (1995) Fluid handling properties of hydrogel dressings. Ostomy Wound Manag 41:54–59

  66. Thomas S, Hughes G, Fram P, Hallett A (2005) An in vitro comparison of the physical characteristics of hydrocolloids, hydrogels, foams and alginate/CMC fibrous dressings. SMTL Report, pp 1–24

  67. Tomic SL, Micic MM, Dobic SN, Filipovic JM, Suljovrujic EH (2010) Smart poly (2-hydroxyethyl methacrylate/itaconic acid) hydrogels for biomedical application. Radiat Phys Chem 79:643–649

  68. Traoré O, Fayard SF, Laveran H (1996) An in-vitro evaluation of the activity of povidone-iodine against nosocomial bacterial strains. J Hosp Infect 34:217–222

  69. Vandeputte J, Hoekstra H (2006) Observed hypergranulation may be related to oedema of granulation tissue. https://www.medline.com/wound-skin-care/derma-gel/lit/Observed%20Hypergranulation.pdf. Accessed 11 Oct 2012

  70. Wapnir RA, Sherry B, Codipilly CN, Goodwin LO, Vancurova I (2008) Modulation of rat intestinal nuclear factor NF-kappaB by gum arabic. Rat small intestine by gum arabic. Dig Dis Sci 53:80–87


  71.  


Acknowledgements


The authors would like to thank Jawaharlal Nehru Hospital, Ajmer for kindly supplying ACD human whole blood used in this work. The authors are also indebted to Department of Biotechnology (DBT), Govt. of India for providing financial support to Laxmi Parwani in terms of fellowship (DBT-JRF/08-09185).


Author Information


Laxmi Parwani
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Newai, India
laxmi_parwani@rediffmail.com
Ashish Bhatnagar
Algae Biofuel and Biomolecules Centre, Department of Microbiology, M.D.S. University, Ajmer, India


Veena Sharma
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Newai, India


Vinay Sharma
Amity Institute of Biotechnology, Amity University,, Jaipur, India