Induction of chromosomal and morphological amelioration in lentil (Lens culinaris Medik.) mutagenized population developed through chemical mutagenesis

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00319-6
First Page: 474
Last Page: 483
Views: 474

Keywords: Lens culinaris , Chemical mutagenesis, Caffeine, Ethyl methane sulphonate, Cytological aberration


Lentil (Lens culinaris Medik.) is an edible pulse crop with an excellent proportion of high-quality protein. The production of lentil should increase worldwide because of its increasing demand and significant economic value. In this present investigation, caffeine and Ethyl methane sulphonate (EMS) were used to induce morphological and cytological variations in M1 generation of lentil. The seeds of lentil L-4076 were treated to five different concentrations (0.10, 0.25, 0.50, 0.75 and 1.0%) of caffeine and EMS to develop viable variants. Various biological parameters (germination, plant survival and pollen fertility), quantitative traits, and cytological studies were performed. These parameters get affected with increasing concentration of mutagens, and sensitivity was found higher in EMS than caffeine. Cytological studies exhibited different types of meiotic abnormalities such as stickiness, laggard, bridge, desynchronisation, disturb polarity, and micronuclei. The frequency of chromosomal aberrations was much higher in the treatment of EMS as compared to caffeine.

                Lens culinaris
              , Chemical mutagenesis, Caffeine, Ethyl methane sulphonate, Cytological aberration

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The authors are thankful to chairman, Department of Botany, Aligarh Muslim University, Aligarh, India for providing facilities for carrying out this study.

Author Information

Sharma Nidhi
Department of Botany, Cytogenetics and Molecular Biology Section, Aligarh Muslim University, Aligarh, India

Shahwar Durre
Department of Botany, Cytogenetics and Molecular Biology Section, Aligarh Muslim University, Aligarh, India

Choudhary Sana
Department of Botany, Cytogenetics and Molecular Biology Section, Aligarh Muslim University, Aligarh, India