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Keywords: n Eucalyptusn , Antifungal activity, Grapevine truck diseases, Botryosphaeriaceae, Hymenochaetaceae
Dieback diseases are caused by Fomitiporia and Botryosphaeria two genus of fungi that colonize wood tissue in grapevine. After the prohibition of sodium arsenite in 2001, no chemical treatments are available to control these diseases. Curettage is a possible substitute solution. The results depend on many factors. The use of essential oils can be an alternative and/or complementary way. This study aims to investigate the essential oil (EO) composition of Eucalyptus globulus (E. globulus) from leaves and flowers and to evaluate its antifungal activity against Botryosphaeria dothidae and Fomitiporia mediterranea fungi. GC–MS results show that 97.79% of oil composition was identified, of which the Eucalyptol is the major compound (76.33%). Other compounds were identified such as α-Pinene (9.81%); Allo-Aromadendrene (3.07%) and Limonene (2.55%). EO had a detrimental effect in vito on both developed Botryosphaeria dothidae and Fomitiporia mediterranea fungi. The effect is dose-dependent and increases with the duration of the treatment. Essential oil concentrations of 0.25% and 0.75% of E. globulus were efficient against Botryosphaeria dothidae and Fomitiporia mediterranea, respectively. This is the first report investigate the purpose of E. globulus EO to control this both phytopathogenic fungi. The results from the antifungal assays reveal that EO of E. globulus has an excellent inhibitory effect against both tested fungi.
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Laboratoire de Recherche Protection Et Valorisation Des Produits Agrobiologique, Departement de Biotechnologie, Faculté Des Sciences de La Nature Et de La Vie, Université Blida-1, Blida, Algeria