Inter simple sequence repeat (ISSR) markers reveal DNA stability in pineapple plantlets after shoot tip cryopreservation

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00327-6
First Page: 360
Last Page: 366
Views: 509

Keywords: Ananas comosus , Cryopreservation, Ex situ conservation, Genetic stability, Molecular markers


Although pineapple (Ananas comosus var. comosus) shoot tips have been cryopreserved but the possible effect of this process at the molecular level has not been studied. This communication describes the growth (plant fresh and dry weights; stem height; leaf length, width and area; and stem base diameter) and the Inter Simple Sequence Repeat (ISSR) analysis of pineapple plantlets of A. comosus MD-2; Red Spanish Florencia; and Hybrid 54 (Smooth Cayenne/Red Spanish) after 45 d of acclimatization. From each of these varieties, the acclimatized plants were obtained from: (1) conventional micropropagation (control 1); (2) from shoot tips submitted to pre-cryostorage conditioning treatments but not exposed to liquid nitrogen (LN) (treatment 2); and (3) from shoot tips exposed to cryostorage including use of LN (treatment 3). The ISSR-PCR method was used to study the genetic stability. There were no statistically significant differences between treatments for the phenotype indicators evaluated. On average, 45 day-old pineapple plants had 0.5 g fresh weight; 1.85 g dry weight; 12.2 cm stem height; 9.1 cm leaf length; 1.6 cm leaf width; 7.1 cm2 leaf area; and 1.4 cm stem base diameter. Also, the potential effects of cryopreservation at the DNA level were not revealed with the eight ISSR markers used, as no polymorphic bands were recorded, which represents 100% genetic stability. As far as we know, this is the first publication on ISSR analysis of pineapple plantlets after cryopreservation.

                Ananas comosus
              , Cryopreservation, Ex situ conservation, Genetic stability, Molecular markers

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Author Information

Villalobos-Olivera Ariel
University of Ciego de Ávila, Ciego de Ávila, Cuba
Ferreira Claudia Fortes
Embrapa Cassava and Fruits, Cruz das Almas, Bahia, Brazil

Yanes-Paz Ermis
Bioplant Centre, University of Ciego de Avila, Ciego de Avila, Cuba

Lorente Gustavo Y.
Bioplant Centre, University of Ciego de Avila, Ciego de Avila, Cuba

Souza Fernanda Vidigal
Embrapa Cassava and Fruits, Cruz das Almas, Bahia, Brazil

Engelmann Florent
IRD, Montpellier, France

Martínez-Montero Marcos Edel
Bioplant Centre, University of Ciego de Avila, Ciego de Avila, Cuba
Lorenzo José Carlos
Bioplant Centre, University of Ciego de Avila, Ciego de Avila, Cuba