MgO nanoparticles mediated seed priming inhibits the growth of lentil (Lens culinaris)

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00400-8
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Keywords: Phytotoxic, Protein digestibility, Antioxidant, Concentration-dependent, Carbohydrate


Abstract


Nanoparticles (NPs) have the potential to influence the growth and development of plants. NPs mediated seed priming is an efficient strategy for crop improvement and agricultural sustainability. However, the effect of NPs on plant growth and development is still not fully understood. Therefore in the present study, the impact of MgO-NPs (10, 50, 100, and 150 μg/ml) on the lentils (Lens culinaris) via nanopriming was determined. The morphological parameters, accumulation of enzymatic, non-enzymatic antioxidants and protein, and carbohydrate content were estimated in response to MgO-NPs priming. The lentils showed 39 and 43% reduction in the shoot and root length, respectively on exposure to 150 μg/ml MgO-NPs as compared to the untreated plants. MgO-NPs induced chlorophyll degradation and a 2–11% reduction in the carbohydrate level. A significant reduction in the flavonoid (27%) and total phenol (80%) accumulation led to a considerable decrease in the antioxidant potential (63%) of nanoprimed lentils than the untreated control. The accumulation and bioavailability of protein was also decreased in MgO-NPs treated lentils. MgO-NPs priming reduced the protein content by 27% and the digestibility by 31% than control lentils. The observed increase in protein precipitable tannins because of reduced carbohydrates was responsible for the evident reduction in protein digestibility and solubility. Overall, the study suggested the potential toxicity of MgO-NPs to lentils in a concentration-dependent manner. However, the observed results cannot be generalized for other crops including legumes. Hence, there is urgent need to investigate the plant responses to MgO-NPs exposure.

Graphical abstract


Phytotoxic, Protein digestibility, Antioxidant, Concentration-dependent, Carbohydrate


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Acknowledgements


PS, AG, and PG are thankful to The Chancellor and The Vice-Chancellor, DAV University, Jalandhar for their continuous support and encouragement to carry out research. VK acknowledges Lovely Professional University management for encouragement.


Author Information


Sharma Priya
Plant Biotechnology and Genetic Engineering Lab, Department of Biotechnology, DAV University, Jalandhar, Punjab, India
psdavuniversity@gmail.com
Gautam Ayushi
Plant Biotechnology and Genetic Engineering Lab, Department of Biotechnology, DAV University, Jalandhar, Punjab, India
agdavuniversity@gmail.com

Kumar Vineet
Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India

vineetkumar22@gmail.com
Guleria Praveen
Plant Biotechnology and Genetic Engineering Lab, Department of Biotechnology, DAV University, Jalandhar, Punjab, India

pvihbt@gmail.com