Molecular tagging of fertility restoration (Rf) gene in sweet pepper (Capsicum annuum var. grossum)

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00343-0
First Page: 903
Last Page: 909
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Keywords: CMS, BSA, Sterility, Fertility, Restorer gene


Abstract


Cytoplasmic male sterility (CMS) is most widely used in the commercial hybrid seed production of chilli and other crop species. It’s a three-line system of hybrid production as it involves a male sterile line, its maintainer and a restorer of fertility for successful hybrid development. Identifying new restorers is much easier in hot pepper as compared to sweet pepper. Almost all the sweet pepper genotypes lack a stable fertility restorer (Rf) allele. Therefore, the identification and development of stable fertility restorer lines with a strong Rf allele is of utmost importance for the commercialization of CMS hybrid production technology in sweet pepper. In the present study, we identified a single dominant gene inheritance pattern of fertility restoration in F2 and backcross populations of sweet pepper lines KTCA5/KTCR15. We also mapped the Rf gene through Bulk segregant analysis of F2 population and based on linkage analysis we identified an SSR marker linked with the male fertility restoration trait.


CMS, BSA, Sterility, Fertility, Restorer gene


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Acknowledgements


We are indebted to Department of science and technology (DST), for providing the fellowship.


Author Information


Nalla Manoj Kumar
Division of Vegetable Science, ICAR-IARI, New Delhi, India
manojhorti@gmail.com
Sharma Vinod K.
NRCPB, ICAR-IARI, New Delhi, India


Srivastava Arpita
Division of Vegetable Science, ICAR-IARI, New Delhi, India


Mangal Manisha
Division of Vegetable Science, ICAR-IARI, New Delhi, India


Rakesh Sharma V.
Division of Vegetable Science, ICAR-IARI, New Delhi, India

Kumar Mukesh
Department of Horticulture, SVPUAT, Meerut, India

Pandav Ajay Kumar
Division of Vegetable Science, ICAR-IARI, New Delhi, India