Manjunatha L., Rani Upasana, Singh Shailendra, Mahesha H. S., Srinivasa N., Kumar Yogesh
Keywords:
Ascochyta blight,
Ascochyta rabiei
, Diversity, RAPD, Pycnidiospore
To study the cultural, morphological and molecular diversity of Ascochyta rabiei infecting chickpea, 44 isolates were sampled from three different agro climatic regions of India over three years (2016–17, 2017–18 and 2018–19). Colony color of the A. rabiei isolates had varied shades of gray and white and produces flat and irregular colonies on potato dextrose agar. The growth rates of isolates varied from 1.02 to 2.46 mm/day. The higher conidial density of 4.05 × 10–6 was recorded in isolate AR-35. Molecular diversity data revealed that out of 58 RAPD markers, 43 markers produced polymorphic band among the 44 isolates with the PIC value ranged from 0.01 to 0.28. Maximum PIC value were revealed by the markers viz., OPA18 (0.2160), OPAU 12 (0.2496), OPB 06 (0.2275) and OPB 09 (0.2805). In cluster analysis isolates were categorized into six different clusters based on 72% similarity. The cluster VI consists of AR-11 and AR-5. The isolate AR-5 found to be diverge from the rest of the isolates and clusters. The cultural, morphological and genetic diversity studies established the existence of diversity and higher gene flow among the isolates of A. rabiei. Mutation and recombination are the main factors for their diversity. The results of the study help in developing resistant cultivars for newly evolving strains of A. rabiei.
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Authors are grateful to the DST-Science and Engineering Research Board (DST-SERB), New Delhi, Government of India (ECR/2016/000855) for financial assistance to carry out this research. Dr. N. P. Singh, The Director, ICAR-IIPR, Kanpur for constant encouragement and support to conduct this research.