Morphological, biochemical and molecular characterization for genetic variability analysis of Capsicum annuum

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00016-5
First Page: 131
Last Page: 141
Views: 1073


Keywords: Chilli, Variability, Genetic advance, Heritability, Correlation, SSR


Abstract

Chilli (Capsicum annuum L.) with immense industrial, therapeutic and export potential is an imperative vegetable and condiment crop of the globe. The genetic variability is plinth for any breeding program. To examine the variability in chilli, 58 chilli genotypes were screened for seven agronomic and five biochemical traits along with 26 SSR markers. The germplasm displayed sufficient variability for all traits at both genotypic and phenotypic levels in consort with more than 60% heritability. Green fruit yield per plant exhibited significant correlation with primary branches per plant (rg = 0.428 and rp = 0.354), fruits per plant (rg = 0.410 and rp = 0.441), single green fruit weight (rg = 0.625 and rp = 0.602), moisture (rg = 0.271 and rp = 0.227) and chlorophyll (rg = 0.382 and rp = 0.368). The path analysis also revealed fruit yield per plant was directly influenced by primary branches per plant (0.115), fruits per plant (0.435), single green fruit weight (0.763), moisture (0.137) and chlorophyll (0.233). Manhattan distance produced 5 clusters, at a limit value of 0.14, during phenotypic based clustering. A total of 73 alleles were generated from 26 SSR primers. The mean alleles per locus were 4. The polymorphic information content (mean = 0.45) confined between 0.17 (CaES4787) to 0.80 (CAMS806). Merely, three groups were generated during molecular based analysis suggesting moderate diversity in the collection. A moderate correlation (0.66) was recorded between the Manhattan’s and Nei’s distance.


Chilli, Variability, Genetic advance, Heritability, Correlation, SSR


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References

  1. Aklilu S, Abebie B, Wogari D, Wolde A (2016) Genetic variability and association of characters in ethiopian hot pepper (Capsicum annum L.) landraces. J Agri Sci 61(1):19–36

  2. Anonymous (2017) http://indianspices.com/sites/default/files/Major%20spice%20wise%20area% 20and%20production%20web%200517.pdf

  3. Bijalwan P, Mishra A (2016) Correlation and path coefficient analysis in chilli (Capsicum annuum) for yield and yield attributing traits. Int J Sci Res 5(3):1589–1592

  4. Boghara MC, Dhaduk HL, Kumar S, Parekh MJ, Patel NJ, Sharma R (2016) Genetic divergence, path analysis and molecular diversity analysis in cluster bean (Cyamopsis tetragonoloba L. Taub.). Ind Crop Prod 89:468–477

  5. Chuichulcherm S, Prommakort S, Srinophakun P, Thanapimmetha A (2013) Optimization of capsaicin purification from Capsicum frutescens Linn with column chromatography using Taguchi design. Ind Crops Prod 44:473–479

  6. Cliffe S, Fawer MS, Maier G, Takata K, Ritter G (1994) Enzyme assays for the phenolic content of natural juices. J Agri Food Chem 42:1824

  7. Dewey JR, Lu KH (1959) A correlation and path coefficient analysis of component of crested wheat seed production. Agron J 51:515–518

  8. Dhaliwal MS, Yadav A, Jindal SK (2014) Molecular characterization and diversity Analysis in chilli pepper using simple sequence repeats (SSR) markers. Afr J Biotech 13(31):3137–3143

  9. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

  10. Farshadfar M, Farshadfar E (2008) Genetic variability and path analysis of chickpea (Cicer arietinum L.) landraces and line. J Appl Sci 8(21):3951–3956

  11. Goldman D, Merril CR (1982) Silver staining of DNA in polyacrylamide gels: linearity and effect of fragment size. Electrophoresis 3:24–26

  12. Hanacek P, Vyhnanek T, Rohrer M, Cieslarova J, Stavelikova H (2009) DNA polymorphism in genetic resources of red pepper using microsatellite markers. J Horti Sci 36(4):127–132

  13. Hasan R, Akand M, Alam N, Bashar A, Mahmudul Huque AKM (2015) Genetic association analysis and selection indices for yield attributing traits in available chilli (Capsicum annuum L.) genotypes. Mol Plant Breed 19(7):1–9

  14. Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57(12):1333–1334

  15. Hossain SM, Habiba U, Bhuyan SI, Haque MS, Begum SN, Hossain DM (2014) DNA fingerprinting and genetic diversity analysis of chilli germplasm using Microsatellite markers. Biotechnology 13:174–180

  16. ICMR (1983) Manual of laboratory techniques. NIN, Hyderabad

  17. Jogi MY, Madalageri MB, Ganiger VM, Bhuvaneswari G, Patil HB, Kotikal YK (2013) Character association and path analysis studies in green chilli (Capsicum annuum L.). Int J Agri Sci 9(2):547–550

  18. Khan MTI, Ali Q, Ashfaq M, Waseem M (2017) Economic analysis of open field chilli (Capsicum annuum L.) Production in punjab, pakistan. J Exp Biol Agric Sci 5(1):121–125

  19. Kumar S, Ramavtar S, Vinod K, Govind V, Abhishek R (2013) Combining molecular-marker and chemical analysis of Capparis decidua (Capparaceae) in the Thar Desert of Western Rajasthan (India). Rev Biol Trop 61:311–320

  20. Martinez S, Mercedes M, Gonzalen-Raurich Bernardo A (2005) The effects of ripening stage and processing systems on vitamin C content in sweet peppers (Capsicum annuum L.). Int Food Sci Nutrition. 56(1):45–51

  21. Maurya AK, Kushwaha ML, Jain SK, Kumar S (2017) Character association and path analysis studies for yield and yield attributes in green chilli (Capsicum annuum L.). Vegetos 30:369–373

  22. Mimura Y, Inoue T, Minamiyama Y, Kubo N (2012) An SSR-based genetic map of pepper (Capsicum annuum) serves as an anchor for the alignment of major pepper maps. Breed Sci 6(2):93–98

  23. Mohammed GH (2013) Effect of seamino and ascorbic Acid on growth, yield and fruits quality of pepper (Capsicum annum L). Int J Pure Appl Sci Tech 17(2):9–16

  24. Mundim GB, Viana JMS, Maia C (2013) Genetic diversity and path analysis for nitrogen use efficiency in popcorn inbred lines. Euphytica 191(2):291–299

  25. Nahak SC, Nandi A, Sahu GS, Tripathy P, Dash SK, Patnaik A, Pradhan SR (2018) Studies on variability, heritability and genetic advance for yield and yield contributing characters in chilli (Capsicum annuum L.). J Pharmacognosy Phytochem 7(1):2506–2510

  26. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc National Acad Sci 70(12):3321–3323

  27. Oh S, Song JY, Lee J, Lee GA, Ko HC, Stoilova T, Krasteva L, Kim YG, Rhee JH, Gwag G, Lee MC (2012) Evaluation of genetic diversity of red pepper landraces (Capsicum annuum L.) from bulgaria using ssr markers. Korean J Int Agri 24(5):547–556

  28. Pacheco-Olvera A, Hernandez-Verdugo S, Rocha-Ramirez V, Gonzalez-Rodriguez A, Oyama K (2012) Genetic diversity and structure of pepper (Capsicum annuum L.) from northwestern Mexico analyzed by microsatellite markers. Crop Sci 52:231–241

  29. Patel DK, Patel BR, Patel J, Kuchhadiya G (2015) Genetic variability and character association studies for green fruit yield and quality component traits in chilli (Capsicum annuum var. longum (DC.) Sendt.). Electronic J Plant Breed 6(2):472–478

  30. Pujar UU, Tirakannanavar S, Jagadeesha RC, Gasti VD, Sandhyarani N (2017) Genetic variability, heritablity, correlation and path analysis in chilli (Capsicum annuum L.). Int J Pure Appl Biosci 5(5):579–586

  31. Quagliotti L, Ottaviano E (1971) Genetic analysis of variability in capsaicin content in two pepper varieties. Genetica Agraria 25:56–66

  32. Rai VP, Kumar R, Kumar S, Rai A, Kumar S, Singh M, Singh SP, Rai AB, Paliwal R (2013) Genetic diversity in Capsicum germplasm based on microsatellite and random amplified microsatellite polymorphism markers. J Physiol Mol Biol Plants 19(4):575–586

  33. Razzaq A, Khan TM, Saeed A, Kamaran S, Zeb A (2016) Genetic diversity and association analysis for different morphological traits in Capsicum annuum. Int J Biomolecules and Biomedicine 5(1):20–28

  34. Rukhsar Patel MP, Parmar DJ, Kalola AD, Kumar S (2017) Morphological and molecular diversity patterns in castor germplasm accessions. Ind Crops Prod 97:316–323

  35. Sharma VK, Semwal CS, Uniyal SP (2010) Genetic variability and character association analysis in bell pepper (Capsicum annuum L.). J Horti For 2(3):58–65

  36. Sharmin A, Hoque ME, Haque MM, Khatun F (2018) Molecular diversity analysis of some chilli (Capsicum spp.) Genotypes using ssr markers. Am J Plant Sci 9:368–379

  37. Singh RB, Singh B, Singh RK (2015) Development of microsatellite (SSRs) markers and evaluation of genetic variability within sugarcane commercial varieties (Saccharum spp. hybrids). Int J Adv Res 3(12):700–708

  38. Singh P, Jain PK, Sharma A (2017) Genetic variability, heritability and genetic advance in chilli (Capsicum annum L.) genotypes. Int J Curr Microbiol Appl Sci 6(9):2704–2709

  39. Sui X, Shan N, Turgeon R, Zhang Z (2017) The complex character of photosynthesis in cucumber fruit. J Exp Bot 68(7):1625–1637

  40. Usman MG, Rafii MY, Ismail MR, Malek MA, Latif MA (2014) Heritability and genetic advance among chili pepper genotypes for heat tolerance and morphophysiological characteristics. Sci World J 1–14

  41. Vaishnavi BA, Khanm H, Bhoomika HR (2017) Correlation and path coefficient analysis in bird’s eye chilli (Capsicum frutescens L.) for yield and yield attributing traits. Int JAgri Sci Res 7(3):259–266

  42. Vidya C, Jagtap VS, Santhosh N (2018) Correlation and path coefficient analysis for yield and yield attributing characters in chilli (Capsicum annum L.) genotypes. Int J Curr Microbiol Appl Sci 7(1):3265–3268

  43. Vyas Govind K, Kumar V, Sharma Sharma R, Sharma RA, Singh JP, Kumar S (2012) Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from the Thar Desert of Rajasthan. Rev de Biol Trop 60(3):1097–1108

  44. Wang D, Bosland PW (2006) The genes of capsicum. HortSci 41(5):1169–1187

  45. Xiao Z, Zheng-Hai Z, Zhen GX, Sheng-li M, Xi-xiang L, Chadœuf J, Palloix A, Li-hao W, Bao-xi Z (2016) Genetic diversity of pepper (Capsicum spp.) germplasm resources in China reflects selection for cultivar types and spatial distribution. J Integrative Agri 15(9):1991–2001

  46. Yaldiz G, Ozguven M, Sekeroglu N (2010) Variation in capsaicin contents of different Capsicum species and lines by varying drying parameters. Ind Crops Prod 32(3):434–438

  47. Yatung T, Dubey R, Singh V, Upadhyay G (2014) Genetic diversity of chilli genotypes of India based on morpho-chemical traits. Aus J of Crop Sci 8(1):97–102

  48.  

  49. Ibarra-Torres P, Valadez-Moctezuma E, Pérez-Grajales M, Rodríguez-Campos J, Jaramillo-Flores ME (2015) Inter- and intraspecific differentiation of Capsicum annuum and Capsicum pubescens using ISSR and SSR markers. Sci Hortic 181:137–146

  50. Liu K, Muse S (2005) Power Marker, an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129

  51. Patel AS, Sasidharan N, Vala AG, Vinay K (2011) Genetic relation in Capsicum annuum L. cultivars through microsatellite markers: SSR and ISSR. Electron J Plant Breed 2:67–76


    52.  


Acknowledgements

Authors acknowledge Anand Agricultural University, Gujarat, India for providing facilities to conduct an experiment.


Author Information

Rahevar Parthsinh M.
Department of Genetics and Plant Breeding, B. A. College of Agriculture, Anand Agricultural University, Anand, India