Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Sustainable biosynthesis and characterizations of silver nanoparticles using Hypoestes phyllostachya Baker leaf extract of Himalayan ranges of India
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Research Articles | Published: 19 May, 2026
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Keywords: Silver nanoparticles, Green synthesis, n Hypoestes phyllostachyan , Antibacterial, Antioxidant, Nanotechnology.
Abstract
Silver nanoparticles (AgNPs) have been synthesized using Hypoestes phyllostachya Baker leaf extract by a green synthesis method. No past research work has been reported on this plant including assessments of silver nanoparticles. The extract used here is a green and natural reducing and capping agent. The synthesis of AgNPs is confirmed by surface plasmon resonance at 424 nm by UV-Visible Spectroscopy. The AgNPs synthesized here have been characterized by FTIR, SEM, EDX, XRD, DLS, and zeta potential measurements, showing spherical shape, crystalline structure, and average particle size of 211.8 nm, and zeta potential of -22.3 mV, indicating moderate stability.The FTIR results confirmed the role of phytochemicals in the reduction and stabilization of AgNPs. The AgNPs showed promising biological activities such as antioxidant activity (IC₅₀ = 215.26 µg/mL by DPPH assay), α-amylase inhibitory activity (IC₅₀ = 144.68 µg/mL), and anti-inflammatory activity (IC₅₀ = 144.29 µg/mL). In addition, the AgNPs synthesized by H. phyllostachya showed promising antibacterial activity against both Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis, and Gram-negative bacteria including Escherichia coli and Pseudomonas aeruginosa in concentration-dependent manner. The results of the present study revealed the potential of H. phyllostachya-mediated AgNPs as promising candidates for biomedical applications.
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Author Information
Department of Pharmaceutical Technology, University of North Bengal, Darjeeling, India