A field study to decipher the role of seed priming and foliar application of plant defence activators on plant growth, disease incidence and yield in bell pepper (Capsicum annuum L.)

*Article not assigned to an issue yet


Research Articles | Published:

E-ISSN: 2229-4473.
Website: www.vegetosindia.org
Pub Email: contact@vegetosindia.org
DOI: 10.1007/s42535-026-01821-5
First Page: 0
Last Page: 0
Views: 83

Keywords: Bell pepper, Plant defence activators, Sustainable agriculture, Disease management


Abstract


Bell pepper production is often constrained by several diseases such as Cercospora leaf spot, anthracnose, bacterial spot, Phytophthora rot, viral and other infections. Extensive use of chemicals to control plant diseases cause environmental pollution, animal and human health risks. This major issue calls for sustainable alternatives for crop production. In this order, plant defence activators could be a viable option for this but their use in agriculture is limited or not fully explored yet. Therefore, to evaluate the effectiveness of plant defence activators, including salicylic acid (SA), jasmonic acid (JA) and β-aminobutyric acid (BABA) as sole and their combination with potassium nitrate (KNO₃) were used in bell pepper. A field trial was conducted at Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, during 2019-20, to optimize foliar application protocols besides seed priming before sowing and seedling dip before transplanting with same plant defence activators to enhance plant resistance against biotic and abiotic stresses while promoting sustainable bell pepper production. Results revealed that all the treatments were significantly superior over the control and among all the treatments SA+KNO3 was the best treatment for plant growth and yield attributes, while BABA treatment was the best to manage plant diseases. The findings contribute to the development of eco-friendly/green approach, reducing chemical dependence and supporting sustainable agricultural practices.

Bell pepper, Plant defence activators, Sustainable agriculture, Disease management


References


Agoncillo EM (2018) Enhancement of Germination and Emergence of Hot Pepper Seeds by Priming with Acetyl Salicylic Acid. J Biol Agric Healthc 8:9–13


AL-surhanee AA (2022) Protective role of antifusarial eco-friendly agents (Trichoderma and salicylic acid) to improve resistance performance of tomato plants. Saudi J Biol Sci 29:2933–2941. https://doi.org/10.1016/j.sjbs.2022.01.020


Ali EA, Mahmoud AM (2013) Effect of foliar spray by different salicylic acid and zinc concentrations on seed yield and yield components of mungbean in sandy soil. Asian J Crop Sci 5:33–40. https://doi.org/10.3923/ajcs.2013.33.40


Ali E, Hussain S, Jalal F, Khan MA, Imtiaz M, Said F, Shah F (2023) Salicylic acid-mitigates abiotic stress tolerance via altering defence mechanisms in Brassica napus (L). Front Plant Sci 14:1187260


El-Naggar AM, El-Nasharty AB A (2016) Effect of Potassium Fertilization on Growth, Flowering, Corms Production and Chemical Contents of Gladiolus hybrida, L. Cv. Alexandria Sci Exch J 37:714–728. https://doi.org/10.21608/asejaiqjsae.2016.2596


Attri K, Sharma A, Sharma M (2024) Protection against Fusarium wilt disease in bell pepper through abiotic resistance inducers. Veg Sci 51:192–195. https://doi.org/10.61180/vegsci.2024.v51.i1.26


Bhutta MUM, Ali H, Hussain S (2023) Seed Priming With Β-Aminobutyric Acid (Baba) Improved Production of Chickpea Genotypes By Optimizing Antioxidant Activity Under Different Moisture Conditions. Appl Ecol Environ Res 21:2863–2880. https://doi.org/10.15666/aeer/2104_28632880


Catoni M, Alvarez-Venegas R, Worrall D et al (2022) Long-Lasting Defence Priming by β-Aminobutyric Acid in Tomato Is Marked by Genome-Wide Changes in DNA Methylation. Front Plant Sci 13:1–13. https://doi.org/10.3389/fpls.2022.836326


Cohen Y, Vaknin M, Mauch-Mani B (2016) BABA-induced resistance: milestones along a 55-year journey. Phytoparasitica 44:513–538. https://doi.org/10.1007/s12600-016-0546-x


Cohen YR (2002) Β-Aminobutyric Acid-Induced Resistance Against Plant Pathogens. Plant Dis 86:448–457. https://doi.org/10.1094/PDIS.2002.86.5.448


Conrath U (2025) Cross-kingdom mechanisms of trained immunity in plant systemic acquired resistance. Nat Plants 11(10):1993–2005


Dewhirst SY, Birkett MA, Loza-Reyes E et al (2012) Activation of defence in sweet pepper, Capsicum annum, by cis-jasmone, and its impact on aphid and aphid parasitoid behaviour. Pest Manag Sci 68:1419–1429. https://doi.org/10.1002/ps.3326


El-Shraiy AM, Hegazi AM (2009) Effect of acetylsalicylic acid, indole-3- bytric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L). Aust J Basic Appl Sci 3:3514–3523


Gomez KN, Gomez AA (1984) Statistical procedures for agricultural research. A Wiley-Inter-Sci. Publication, vol 2. Wiley, New York, p 68


Härdter R (1997) Crop nutrition and plant health of rice based cropping systems in Asia. Agro-Chemicals News Br. eng, vol 20 pp. 29–39


Holzmueller EJ, Jose S, Jenkins MA (2007) Influence of calcium, potassium, and magnesium on Cornus florida L. density and resistance to dogwood anthracnose. Plant Soil 290:189–199. https://doi.org/10.1007/s11104-006-9151-y


Jakab G, Cottier V, Toquin V et al (2001) β-Aminobutyric acid-induced resistance in plants. Eur J Plant Pathol 107:29–37. https://doi.org/10.1023/A:1008730721037


Kabir MY (2019) Abiotic factors affecting plant physiology and fruit yield and. University of Georgia


Kazemi M (2014) Effect of foliar application with salicylic acid and methyl jasmonate on growth, flowering, yield and fruit quality of tomato. Bull Environ Pharmacol Life Sci 3:154–158


Khan MIR, Poor P, Janda T (2022) Salicylic Acid: A Versatile Signaling Molecule in Plants. J Plant Growth Regul 41:1887–1890. https://doi.org/10.1007/s00344-022-10692-4


Khalili L (2026) Fungal Delignification and Its Impact on Biochemical Pathways in the Plant Defence System. Molecular Mechanisms in Host-Fungal Interactions. Springer Nature Singapore, Singapore, pp 247–271


Khandaker L, Masum Akond A, Oba S (2011) Foliar application of salicylic acid improved the growth, yield and leaf’s bioactive compounds in red amaranth (Amaranthus tricolor L). Veg Crop Res Bull 74:77–86. https://doi.org/10.2478/v10032-011-0006-6


Khodary SE (2009) Effect of Salicylic Acid on the Growth and Mineral nutrition in Salt Stressed Wheat Plants. Int J Agric Biol 19:177–180


Kim JY, Kang HW (2023) β-aminobutyric acid and powdery mildew infection enhanced the activation of defence-related genes and salicylic acid in cucumber (Cucumis sativus L). Genes. https://doi.org/10.3390/genes14112087


Kim YC, Kim YH, Lee YH et al (2013) Β-Amino-N-Butyric Acid Regulates Seedling Growth and Disease Resistance of Kimchi Cabbage. Plant Pathol J 29:305–316. https://doi.org/10.5423/PPJ.OA.12.2012.0191


Koo YM, Heo AY, Choi HW (2020) Salicylic acid as a safe plant protector and growth regulator. Plant Pathol J 36:1–10. https://doi.org/10.5423/PPJ.RW.12.2019.0295


Kouser S, Rehaman A, Ahmed S et al (2022) Crosstalk of potassium and phytohormones under abiotic stress. Role Potassium Abiotic Stress 89–110. https://doi.org/10.1007/978-981-16-4461-0_5


Kwiatkowski CA (2012) Response of winter rape (Brassica napus L. ssp. oleifera Metzg., Sinsk) to foliar fertilization and different seeding rates. Acta Agrobot 65:161–170. https://doi.org/10.5586/aa.2012.070


Larqué-Saavedra A, Martin-Mex R (2007) Effects of salicylic acid on the bioproductivity of plants. Salicylic Acid: A Plant Hormone. Springer, Dordrecht, pp 15–23. https://doi.org/10.1007/1-4020-5184-0


Li A, Sun X, Liu L (2022) Action of Salicylic Acid on Plant Growth. Front Plant Sci 13. https://doi.org/10.3389/fpls.2022.878076


Li J, Cai B, Chang S et al (2023) Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid. Front Plant Sci 14:1195932. https://doi.org/10.3389/fpls.2023.1195932


Mahajan G, Sarlach RS, Gill MS (2012) Effect of foliar application of potassium nitrate and urea on performance of transplanted rice. Eco Env Conserv 18:533–534


Mamphogoro TP, Babalola OO, Aiyegoro OA (2020) Sustainable management strategies for bacterial wilt of sweet peppers (Capsicum annuum) and other Solanaceous crops. J Appl Microbiol 129:496–508. https://doi.org/10.1111/jam.14653


Mandavia MK, Khan NA, Gajera HP et al (2000) Inhibitory effects of phenolic compounds on fungal metabolism in host-pathogen interactions in Fusarium wilt of cumin. Allelopath J 7:85–92


Marschner H (2011) Marschner’s mineral nutrition of higher plants. Academic


Mckinney H (1923) A new system of grading plant diseases. J agricultural Res 26:195–218 J Agric Res 26:195–218


Mengel K, Kirkby EA (2001) Principles of plant nutrition. 5th edidtion ed. Dordr Springer https//doi org/10 1007:978–994


Menjell K (1976) Potassium in plant physiology and yield formation. Indian Soc Soil Sci Bull 10:23–40


Mishra S, Roychowdhury R, Ray S et al (2024) Salicylic acid (SA)-mediated plant immunity against biotic stresses: An insight on molecular components and signaling mechanism. Plant Stress 11:100427. https://doi.org/10.1016/j.stress.2024.100427


Morales-Martinez M, Moscoso-Ramírez PA, Castelán-Estrada M, Contreras- Oliva A (2020) Effect of nitrates alone or with paclobutrazol on flowering induction and production in mango cv. Tommy Atkins//Efecto de nitratos solos o con paclobutrazol en la inducción floral y producción en mango cv. Tommy Atkins Biotecnia 22:20–27. https://doi.org/10.18633/biotecnia.v22i2.1242


Mulaudzi T, Sias G, Nkuna M et al (2023) Seed Priming with MeJa Prevents Salt-Induced Growth Inhibition and Oxidative Damage in Sorghum bicolor by Inducing the Expression of Jasmonic Acid Biosynthesis Genes. Int J Mol Sci 24. https://doi.org/10.3390/ijms241210368


Nego J, Dechassa N, Dessalegne L (2015) IJCST Effect of Seed Priming with Potassium Nitrate on Bulb Yield and Seed Quality of Onion (Allium Cepa L.), under Rift Valley Conditions, Central Ethiopia. Int J Crop Sci Technol 1:1–12


Nkrumah P, Amadu AM, Ayeh KO (2021) Influence of salicylic acid and potassium nitrate on plant height and flowering time of groundnut (Arachis hypogaea L.) under varying salinity and drought-induced stresses. Ghana J Sci 62:26–36. https://doi.org/10.4314/gjs.v62i1.3


Nyandwi J, Ghimire A, Khan W et al (2024) Journal of A gricultural, L ife and E nvironmental S ciences Effect of Potassium Nitrate Priming in the Germination and Early Seedling Growth of Soybean. 36:332–347. https://doi.org/10.22698/jales.20240025


Pal P, Ansari SA, Jalil SU, Ansari MI (2023) Regulatory role of phytohormones in plant growth and development. Plant hormones in crop improvement. Academic, pp 1–13


Prabhu AS, Fageria NK, Huber DM, Rodriguis FÁ (2007) Potassium and Plant Disease. In: Datnoff LE, WH E, DM H (eds) Mineral nutrition and plant disease. American Phytopathological Society, Saint Paul, pp 57–78


Rajjou L, Belghazi M, Huguet R et al (2006) Proteomic investigation of the effect of salicylic acid on arabidopsis seed germination and establishment of early defence mechanisms. Plant Physiol 141:910–923. https://doi.org/10.1104/pp.106.082057


Ren X, Wang J, Zhu F et al (2022) β-aminobutyric acid (BABA)-induced resistance to tobacco black shank in tobacco (Nicotiana tabacum L). PLoS ONE 17:267960. https://doi.org/10.1371/journal.pone.0267960


Sanatombi K (2023) Antioxidant potential and factors influencing the content of antioxidant compounds of pepper: A review with current knowledge. Compr Rev Food Sci Food Saf 22:3011–3052. https://doi.org/10.1111/1541-4337.13170


Sangha MK, Atwal AK, Sandhu PS et al (2007) Salicylic acid induces resistance to Alternaria blight in crop Brassica species. In: Proceedings The 12th International Rapeseed Congress IV Sustainable Development in Cruciferous Oilseed Crops Production Wuhan. China March, pp 26–30


Sarkar RK, Malik GC (2001) Effect of foliar spray of potassium nitrate and calcium nitrate on grasspea (Lathyrus sativus L.) grown in rice fallows. Jointly Support by 2:47


Shakirova FM, Sakhabutdinova AR, Bezrukova MV et al (2003) Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Sci 164:317–322. https://doi.org/10.1016/S0168-9452(02)00415-6


Shareef HJ (2019) Salicylic acid and potassium nitrate promote flowering through modulating the hormonal levels and protein pattern of date palm Phoenix dactylifera Sayer offshoot. Acta Agric Slov 114:231–238. https://doi.org/10.14720/aas.2019.114.2.8


Sharma P, Sardana V, Banga SS (2013) Effect of salicylic acid on growth and seed filling in Indian mustard (Brassica juncea L.) under high temperature stress. Vegetos 26:243–248. https://doi.org/10.5958/j.2229-4473.26.1.035


Shinde AK, Jamadagni BM, Birari SP (1991) Effect of foliar spray of growth regulators and KNO3 on growth and yield of cowpea (Vigna unguiculata L. Walp) variety VCM-8. Indian J Plant Physiol 24:392–395


Sreedhara DS, Kerutagi MG, Basavaraja H et al (2013) Economics of capsicum production under protected conditions in Northern Karnataka *. Karnataka J Agric Sci 26:217–219


Tak Y, Kaur M, Gautam C, Kumar R, Tilgam J, Natta S (2023) Phenolic biosynthesis and metabolic pathways to alleviate stresses in plants. Plant phenolics in abiotic stress management. Springer Nature Singapore, Singapore, pp 63–87


Thomson T, Patel GS, Thakar JB, Pandya KS (2017) Effect of Foliar Application of Acetyl Salicylic Acid and Ascorbic Acid on Protein Content, Yield and Economics of Garden Pea (Pisum sativum L.) cv. Bonneville. Int J Curr Microbiol Appl Sci 6:1987–1990. https://doi.org/10.20546/ijcmas.2017.606.233


Verma A, Singh D, Singh D, Mittal N (2026) Microbe-Induced Systemic Resistance: A Promising Avenue for Disease Management in Agriculture. Plant–Microbe Interactions for Sustainable Growth and Resilience. CRC, pp 262–280


Yazdanpanah M, Maki H, Bakhtiari I et al (2015) Effect of salicylic acid, nano-iron chelate and pseudomonas on quality and quantity of rapeseed yield. J Bio Env Sci 310:310–317




 


Author Information


Faculty of Agriculture, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, India