Ocimum is an aromatic plant species with immense medicinal properties. It contains several bioactive compounds that are used in the preparation of synthetic drugs. The study is aimed to compare the volatile components of in-vitro raised plants with the donor mother plant. The in-vitro regenerated plants showed 52 volatile compounds, whereas the mother plant showed 41 compounds only. It was observed that in-vitro regenerants consisted of a higher percentage of volatiles (38.92%) as compared to the mother plant (28.8%). Many similar constituents were identified from both the mother plant and in-vitro raised plants such as linalool, camphor, and δ-terpineol. However, some constituents were obtained from in-vitro raised plants only such as α-cubebene, β-chamigrene, trans-calamenene, cubebol, spathulenol, isogermacrene D, cedrol, isocitronellol, cheloviolene D, cis-β-terpeniol, thujol, γ-amorphene, γ-gurjunene, and γ-elemene. Two diterpenes (neocembrene and cheloviolene D) were also present in the in-vitro raised plants. The current study provides an alternative approach for quick synthesis of bioactive products of medicinal and other commercial use.
The authors are thankful to Prof. V. P. Singh, former Heads of the Department of Botany, University of Delhi, for providing facilities to conduct the research work. We also extend our thanks to the Advanced Instrumentation Research Facility (AIRF), Jawahar Lal Nehru University, New Delhi for GC–MS analysis. Authors are also thankful to Principal BCAS, University of Delhi. Indira Gandhi National Open University, Maidan Garhi, New Delhi is gratefully acknowledged for providing fellowship to Anamika Tripathi.