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Keywords: n Blumea balsamiferan , Ie-Brôuk, Ie-Jue, Ie-Seu’um, Seulawah Agam, n Strepblus aspern
This study investigates the phytochemical composition and antioxidant activity of medicinal plants growing in three geothermal areas of the Seulawah Agam Mountains in Aceh Province, Indonesia: Ie-Brôuk, Ie-Jue, and Ie-Seu’um. A total of fifteen plant species from ten families were evaluated. Phytochemical screening revealed that tannins are the most ubiquitous secondary metabolites, suggesting their key role in plant adaptation to geothermal environments. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, with IC₅₀ values determined from dose–response curves and linear regression models. The three most active species based on DPPH-scavenging activity were Streblus asper (34.80 ppm), Antidesma montanum (35.21 ppm), and Tephrosia noctiflora (43.77 ppm). Among the areas, Ie-Jue showed the highest overall effectiveness, followed by Ie-Seu’um and Ie-Brôuk, with corresponding R2 values of 0.990, 0.942, and 0.750, indicating strong model fit. These results suggest that DPPH-based dose–response curves provide a reliable measure of antioxidant potential in geothermal-adapted medicinal plants.
Abubakar A, Yusuf H, Syukri M et al (2023) Heavy metals contamination in geothermal medicinal plant extract (Chromolaena odorata Linn). Glob J Environ Sci Manag 9:995–1004. https://doi.org/10.22035/gjesm.2023.04.22
Akaike H (1974) A new look at the statistical model identification. IEEE Trans Automat Contr 19:716–723. https://doi.org/10.1109/TAC.1974.1100705
Altemimi A, Lakhssassi N, Baharlouei A et al (2017) Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. https://doi.org/10.3390/plants6040042
Anadozie SO, Aduma AU, Adewale OB (2024) Alkaloid-rich extract of Buchholzia coriacea seed mitigate the effect of copper-induced toxicity in Drosophila melanogaster. Vegetos 37:460–468. https://doi.org/10.1007/s42535-023-00760-9
Das AK, Islam MN, Faruk MO et al (2020) Review on tannins: extraction processes, applications and possibilities. S Afr J Bot 135:58–70. https://doi.org/10.1016/j.sajb.2020.08.008
Elekofehinti OO (2015) Saponins: anti-diabetic principles from medicinal plants—a review. Pathophysiology 22:95–103. https://doi.org/10.1016/j.pathophys.2015.02.001
Fakri F, Harahap SP, Muhni A et al (2023) Antimicrobial properties of medicinal plants in the lower area of Ie Seu-um geothermal outflow, Indonesia. Malacca Pharm 1:55–61
Funk VA, Bayer RJ, Keeley S et al (2005) Everywhere but Antarctica: using a supertree to understand the diversity and distribution of the compositae. Biol Skr 55:343–373
Harborne JB (1987) Phytochemical methods: a guide to modern techniques of plant analysis. Springer, New York
Harera CF, Maysarah H, Kemala P et al (2024) Geothermal flora and AgNPs synergy: a study on the efficacy of Lantana camara and Acrostichum aureum-infused hand sanitizers. Grimsa J Sci Eng Technol 2:52–59. https://doi.org/10.61975/gjset.v2i2.38
Idroes R, Yusuf M, Saiful S et al (2019) Geochemistry exploration and geothermometry application in the North Zone of Seulawah Agam, Aceh Besar district. Indonesia Energies (Basel) 12:4442. https://doi.org/10.3390/en12234442
Idroes GM, Khairan K, Suhartono E et al (2025) Resilience and adaptation: plant ecology in Indonesia’s geothermal environments. Leuser J Environ Stud 3:44–55. https://doi.org/10.60084/ljes.v3i1.294
Kemala P, Idroes R, Khairan K et al (2022) Green synthesis and antimicrobial activities of silver nanoparticles using Calotropis gigantea from Ie Seu-Um Geothermal Area, Aceh Province. Indonesia Mol 27:5310. https://doi.org/10.3390/molecules27165310
Khairan K, Maulydia NB, Faddillah V et al (2024) Uncovering anti-inflammatory potential of Lantana camara Linn: Network pharmacology and in vitro studies. Narra J 4:1–16. https://doi.org/10.52225/narra.v4i2.894
Lala A, Yusuf M, Suhendra R et al (2024) Characterization of geochemical and isotopic profiles in the southern zone geothermal systems of Mount Seulawah Agam, Aceh Province, Indonesia. Leuser J Environ Stud 2:30–40. https://doi.org/10.60084/ljes.v2i1.172
Letchuman S, Madhuranga HDT, Kaushalya MBLN et al (2024) Alkaloids unveiled: a comprehensive analysis of novel therapeutic properties, mechanisms, and plant-based innovations. Intelligent Pharm. https://doi.org/10.1016/j.ipha.2024.09.007
Liu W, Yin D, Li N et al (2016) Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and its quality assessment. Sci Rep 6:1–18. https://doi.org/10.1038/srep28591
Lourenço SC, Mold M, Alves VD (2019) Antioxidants of natural plant origins: from sources to food industry applications. Molecules. https://doi.org/10.3390/molecules24224132
Lyles RH, Poindexter C, Evans A et al (2008) Nonlinear model-based estimates of IC50 for studies involving continuous therapeutic dose-response data. Contemp Clin Trials 29:878–886. https://doi.org/10.1016/j.cct.2008.05.009
Maulydia NB, Khairan K, Tallei TE et al (2023) GC-MS analysis reveals unique chemical composition of Blumea balsamifera (L.) DC in Ie-Jue geothermal area. Grimsa J Sci Eng Technol 1:9–16. https://doi.org/10.61975/gjset.v1i1.6
Maulydia NB, Khairan K, Tallei TE et al (2024b) Exploring the medicinal potential of Blumea balsamifera insights from molecular docking and molecular dynamics simulations analyses. Malacca Pharm 2:33–40. https://doi.org/10.60084/mp.v2i1.168
Maulydia NB, Idroes R, Khairan K, Tallei TE (2025) Phytochemical analysis and antioxidant activity of two Phyllanthaceae family plants from Ie-Brôuk geothermal area. IOP Conf Ser Earth Environ Sci 1477:012030. https://doi.org/10.1088/1755-1315/1477/1/012030
Maulydia NB, Khairan K, Tallei TE et al (2024a) Analysis of geothermal impact on metabolite compounds of heat-tolerant plant species using clustering and similarity cliff. Global J Environ Sci Manag 10:1–16. https://doi.org/10.22034/gjesm.2024.04
Narayanan S, Prasad PVV, Welti R (2018) Alterations in wheat pollen lipidome during high day and night temperature stress. Plant Cell Environ 41:1749–1761. https://doi.org/10.1111/pce.13156
Owoyele VB, Adediji JO, Soladoye AO (2005) Anti-inflammatory activity of aqueous leaf extract of Chromolaena odorata. Inflammopharmacology 13:479–484. https://doi.org/10.1163/156856005774649386
Phaniendra A, Jestadi DB, Periyasamy L (2015) Free radicals: properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 30:11–26. https://doi.org/10.1007/s12291-014-0446-0
Ritz C, Baty F, Streibig JC, Gerhard D (2015) Dose-response analysis using R. PLoS ONE 10:1–13. https://doi.org/10.1371/journal.pone.0146021
Rolnik A, Olas B (2021) The plants of the Asteraceae family as agents in the protection of human health. Int J Mol Sci 22:1–10. https://doi.org/10.3390/ijms22063009
Roy A, Khan A, Ahmad I et al (2022) Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. Biomed Res Int. https://doi.org/10.1155/2022/5445291
Stout RG, Al-Niemi TS (2002) Heat-tolerant flowering plants of active geothermal areas in Yellowstone National Park. Ann Bot 90:259–267. https://doi.org/10.1093/aob/mcf174
Suriyatem R, Auras RA, Intipunya P, Rachtanapun P (2017) Predictive mathematical modeling for EC50 calculation of antioxidant activity and antibacterial ability of Thai bee products. J Appl Pharm Sci 7:122–133. https://doi.org/10.7324/JAPS.2017.70917
U.S. Department of Agriculture ARS (1992) Dr. Duke’s Phytochemical and Ethnobotanical Databases. http://phytochem.nal.usda.gov/
Zargoosh Z, Ghavam M, Bacchetta G, Tavili A (2019) Effects of ecological factors on the antioxidant potential and total phenol content of Scrophularia striata Boiss. Sci Rep 9:1–15. https://doi.org/10.1038/s41598-019-52605-8
Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia