Kushwaha Prem Prakash, Kumar Ramesh, Neog Panchi Rani, Behara Malay Ranjan, Singh Pratibha, Kumar Ajay, Prajapati Kumari Sunita, Singh Atul Kumar, Shuaib Mohd, Sharma Amit Kumar
Keywords: Ayurvedic product, Phytochemicals, Cancer, Oxidative stress, Free radical
In the present comparative study, the authors studied the antioxidant and anticancer activity of commercially available polyherbal Indian Ayurvedic products namely Divya Sarvakalp Kwath (DSKK), Divya Sanjivani Vati (DSV), Kanchanar Guggulu (KG) and Shakti Drop (SD). Authors also quantified phenolic and flavonoid contents in the samples. Solid powdered samples (DSKK, DSV, and KG) were extracted in methanol and water (1:1) using cold extraction method. Spectrophotometry technique was used to quantify the phytochemicals present in test samples. DSKK showed comparatively higher content of total phenolics (247.65 ± 0.05 μgPGE/g) and flavonoid (34.66 ± 0.19 µgQE/mg). Radical scavenging, metal ion chelation and reducing potential of test products were studied using nitric oxide scavenging, DPPH, metal ion chelation, reducing power ability, and phosphomolybdate in vitro antioxidant assays at different concentration. Dose-dependent antioxidant activity was observed in all the test samples at 100–500 µg or µl/ml concentration. Anticancer efficacy of the test samples were studied in lung (A549), colon (Colo205), and breast cancer (MCF7) cell lines at different concentrations (10–100 µg or µl/ml) using MTT assay. Confocal microscopy was used to reveal the apoptotic induction, mitochondrial membrane integrity disruption and reactive oxygen species production ability of test products in cancer cells. The present study revealed that DSKK possesses comparatively better antioxidant potential and SD has potent anticancer activity against breast cancer cells.
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PPK acknowledges financial support from the Indian Council of Medical Research as Senior Research fellowship. SK acknowledges University Grants Commission, India and Department of Science and Technology, India for providing financial support in the form of UGC-BSR Research Start-Up-Grant [No. F.30–372/2017 (BSR)] and DST-SERB Grant [EEQ/2016/000350] respectively. SK acknowledges DST-FIST India, for providing financial support to the Department of Biochemistry, Central University of Punjab, Bathinda, India. KSP, AKS, MS acknowledges Department of Biotechnology, Council of Scientific and Industrial Research, and Indian Council of Medical Research, India respectively for research fellowships.