Characterization of phytochemicals and validation of antioxidant and anticancer activity in some Indian polyherbal ayurvedic products

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00205-1
First Page: 286
Last Page: 299
Views: 735


Keywords: Ayurvedic product, Phytochemicals, Cancer, Oxidative stress, Free radical


Abstract

In the present comparative study, the authors studied the antioxidant and anticancer activity of commercially available polyherbal Indian Ayurvedic products namely Divya Sarvakalp Kwath (DSKK), Divya Sanjivani Vati (DSV), Kanchanar Guggulu (KG) and Shakti Drop (SD). Authors also quantified phenolic and flavonoid contents in the samples. Solid powdered samples (DSKK, DSV, and KG) were extracted in methanol and water (1:1) using cold extraction method. Spectrophotometry technique was used to quantify the phytochemicals present in test samples. DSKK showed comparatively higher content of total phenolics (247.65 ± 0.05 μgPGE/g) and flavonoid (34.66 ± 0.19 µgQE/mg). Radical scavenging, metal ion chelation and reducing potential of test products were studied using nitric oxide scavenging, DPPH, metal ion chelation, reducing power ability, and phosphomolybdate in vitro antioxidant assays at different concentration. Dose-dependent antioxidant activity was observed in all the test samples at 100–500 µg or µl/ml concentration. Anticancer efficacy of the test samples were studied in lung (A549), colon (Colo205), and breast cancer (MCF7) cell lines at different concentrations (10–100 µg or µl/ml) using MTT assay. Confocal microscopy was used to reveal the apoptotic induction, mitochondrial membrane integrity disruption and reactive oxygen species production ability of test products in cancer cells. The present study revealed that DSKK possesses comparatively better antioxidant potential and SD has potent anticancer activity against breast cancer cells.


Ayurvedic product, Phytochemicals, Cancer, Oxidative stress, Free radical


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Acknowledgements

PPK acknowledges financial support from the Indian Council of Medical Research as Senior Research fellowship. SK acknowledges University Grants Commission, India and Department of Science and Technology, India for providing financial support in the form of UGC-BSR Research Start-Up-Grant [No. F.30–372/2017 (BSR)] and DST-SERB Grant [EEQ/2016/000350] respectively. SK acknowledges DST-FIST India, for providing financial support to the Department of Biochemistry, Central University of Punjab, Bathinda, India. KSP, AKS, MS acknowledges Department of Biotechnology, Council of Scientific and Industrial Research, and Indian Council of Medical Research, India respectively for research fellowships.


Author Information

Kushwaha Prem Prakash
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

Kumar Ramesh
Department of Biochemistry, University of Allahabad, Prayagraj, India


Neog Panchi Rani
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India


Behara Malay Ranjan
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India


Singh Pratibha
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

Kumar Ajay
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

Prajapati Kumari Sunita
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

,
Singh Atul Kumar
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

Shuaib Mohd
Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, India

Sharma Amit Kumar
Department of Biochemistry, University of Allahabad, Prayagraj, India