Vegetos
(An International Journal of Plant Research & Biotechnology)
Comparative study of ambiguity in market collected Sariva sample using HPTLC and antioxidant assay
Research Articles | Published: 10 December, 2024
First Page: 79
Last Page: 86
Views: 1839
Keywords: Sariva, Vanillin, HPTLC, Antioxidant potential, Ambiguity
Abstract
The plant species Sariva is highly noteworthy in Ayurveda due to its diverse range of applications. Vanillin is major active constituent reported in drug Sariva. Nevertheless, the task of recognizing and procuring genuine Sariva species is hindered by the presence of other plant materials in market. These other plant materials may be mistakenly sold as Sariva or mixed with genuine Sariva, making it difficult for consumers to obtain the authentic drug. This poses a challenge for both Ayurvedic practitioners and individuals seeking specific benefits of Sariva. An examination was undertaken to determine the effectiveness of selected samples of Sariva from major markets in India by assessing their antioxidant capabilities, ability to scavenge free radicals, and qualitative assessment of vanillin as an active phytoconstituent. To analyse the concentration of vanillin, a mobile phase consisting of chloroform, ethyl acetate, methanol, and formic acid in a ratio of 1.5:7:1:0.5 was used to achieve optimal separation. The spectral analysis was conducted at a wavelength of 254 nm, and the calibration curves exhibited a linear relationship within the range of 400–1800 ng per spot. The findings revealed that methanolic extract of ‘Sariva’ acquired from different markets exhibited a greater variation in the concentration of vanillin. Furthermore, the antioxidant capacity of selected ‘Sariva’ extracts demonstrated considerable variation in antioxidant efficacy, suggesting the remarkable ambiguity in marketed samples.
References
Aiyer KN (1951) Pharmacognosy of ayurvedic drugs of travancore. Cochin. Central Res Institute 1:1
Aneja V, Suthar A, Verma S, Kalkunte S (2008) Phyto-pharmacology of Hemidesmus indicus (L.) R. Br. Pharm Rev 1:143
Anwar F, Manzoor M, Bajwa J (2004) Antioxidant activity of solvent extracts of strawberry using various antioxidant assay. Pak J Anal Chem 5(2):2837
Austin A (2008) A Review on Indian sarsaparilla, Hemidesmus indicus (L.) R. Br. J Biol Sci 1:1–12. https://doi.org/10.3923/jbs.2008.1.12
Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol. https://doi.org/10.1111/j.1751-1097.1999.tb01944.x
Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B (2011) Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Res Int. https://doi.org/10.1016/j.foodres.2009.09.003
De Pasquale A (1984) Pharmacognosy: the oldest modern science. J Ethnopharmacol 11:116
Firn RD, Jones CG (2003) Natural products – a simple model to explain chemical diversity. Nat Prod Rep. https://doi.org/10.1039/B208815K
Ganskopp D, Bohnert D (2003) Mineral concentration dynamics among 7 northern Great Basin grasses. J Range Manage. https://doi.org/10.2307/4003902
Gupta MM, Verma RK, Misra LN (1992) Terpenoids from Hemidesmus indicus (L.) R. Br. Phytochemistry. https://doi.org/10.1016/S0031-9422(00)97582-4
Hussain J, Khan AL, Rehman N, Zainullah HST, Khan F et al (2009) Proximate and nutrient analysis of selected medicinal plant species of Pakistan. Pak J Nutr 8(5):620–624. https://doi.org/10.1016/S2221-1691(12)60390-6
Iqbal S, Bhanger MI (2006) Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Comp Anal 19:544–551. https://doi.org/10.1016/j.jfca.2005.05.001
Kaplan F, Kopka J, Sung DY, Zhao W, Popp M, Porat R, Guy CL (2007) Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content. Plant J 50:967–981. https://doi.org/10.1111/j.1365-313x.2007.03100.x
Khan ZI, Ashraf M, Valeem EE (2006) Forage mineral status evaluation-the influence of pastures. Pak J Bot 38:10431054
Krishnarajua AV, Rao TVN, Sundararajua D, Vanisreeb M, Tsayb H, Subbaraju GV (2005) Assessment of bioactivity of India medicinal plants using Brine Shrimp (Artemia salina) Lethality Assay. Int J Appl Sci Eng. 3(2):125–134
Kumar S, Budhwar L, Yadav A, Yadav M, Yadav JP (2016) Phytochemical screening and antibacterial activity of Aloe vera collected from different climatic regions of India. Nat Prod J 6:73–82. https://doi.org/10.1186/s131040172385-3
Liu Y, Qian C, Ding S, Shang X, Yang W, Fang S et al (2016) Effect of light regime and provenance on leaf characteristics, growth and flavonoid accumulation in Cyclocarya paliurus (Batal) Iljinskaja coppices. Bot Stud 28:1–13. https://doi.org/10.1186/s40529-016-01457
PrasadS WSP, Mishra LC (1964) Pharmacognostical investigation on Indian Sarasaparilla, its substitutes and adulterants Part I, Hemidesmus indicus R. Br. Indian J Pharm 81:1–12. https://doi.org/10.3923/jbs.2008.1.12
Sanders TH (1982) Peanut triacylglycerols: effect of season and production location. J Am Oil Chem Soc 59(8):346–351. https://doi.org/10.1007/BF02541017
Warrier PK, NambiarVPK GPM (2000) Some important medicinal plants of the Western ghats, India- A profile. Int Dev Res Centre 11:935. https://doi.org/10.1111/j.2042-7158.1994.tb05722.x
Zhang Y, Xi W, Sun Y, Shen Y, Ye X, Zhou Z (2014) Phenolic composition of chinese wild mandarin (Citrus reticulata Balnco.) pulps and their antioxidant properties. Ind Crops Prod 52:466–474. https://doi.org/10.1016/j.indcrop.2013.11.016
Author Information
Department of Plant Biotechnology, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth (Deemed to Be University), Pune, India