Vegetos
(An International Journal of Plant Research & Biotechnology)
Illigera Grandiflora W.W. Sm. & Jeffrey (Hernandiaceae): a potent source of antioxidants and in silico molecular docking analysis as vascular endothelial growth factor (VEGF) pathway regulator
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Pathak Ashutosh, Das Rahul, Vijayan Deepu, Odyuo Nripemo, Baite David Lalsama, Mao Ashiho Asosii
Research Articles | Published: 21 February, 2025
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Keywords: Illigera grandiflora, LC-MS, Pharmacokinetics, pkCSM
Abstract
New insights into the drug discovery and development were based on the traditional knowledge. Tubers of Illigera grandiflora were used traditionally for the treatment of dropsy and traumatic injury in Traditional Chinese Medicine while ethnomedicinal knowledge present in Khiamniungan tribe, Nagaland, India reveals the role of I. grandiflora leaves in wound healing. In the present study, Illigera grandiflora W.W. Sm. & Jeffrey (Hernandiaceae) was evaluated for phytochemical compounds, antioxidant properties, detection of active compounds and probable mechanisms of action in wound healing. The methanolic leaf extract exhibited promising results in three antioxidants assays viz. DPPH, ABTS and Reducing Power Assay, compared to ascorbic acid as standard. (-)-Grandiflorimine/(-)-Grandifloramine, Methyl Vanillate, Ovigerine, Reticuline and Roemrefidine were identified through LC-MS. Reticuline (isoquinoline alkaloid) exhibited the most potent pharmacokinetic properties based on pkCSM models. The probable molecular mechanism of mode of action in present study was investigated through the potential of reticuline in inhibiting the HIF-1alpha degrading prolyl hydroxylase (PHDs). PHDs degrades the HIF-1 alpha under normoxia condition while under hypoxia PHDs are deactivated resulting into the activation of vascular endothelial growth factor (VEGF) pathway by HIF-1alpha. Reticuline exhibits the potential of binding PHDs thus which may trigger VEGF pathway under normoxia conditions leading to several important physiological processes such as vasodilation of blood vessels, increases permeability of blood vessel wall, and promotion of growth and proliferation of endothelial cells that lines blood vessels. Medicinal properties such as analgesic, CNS stimulant, Dopamine receptor blocking effect, and many more were already associated and reported for reticuline suggesting in multiple mode of molecular mechanisms.
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Author Information
Forest Botany Division, Forest Research Institute, Dehradun, India