Vegetos
(An International Journal of Plant Research & Biotechnology)
Taxonomic delineation of genus Clausena Burm. f. with special emphasis on foliar macro and micromorphology
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Research Articles | Published: 17 June, 2025
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Keywords: Assam, n Clausenan , Foliar, Palynology, Stomata, Taxonomy
Abstract
Clausena is well known genus under the family Rutaceae and most of the plants under the genus are aromatic and used in traditional medicine. The present study was aimed to investigate the foliar macro and micromorphology (epidermis, stomata, trichome), leaf venation pattern and palynology of three species of Clausena viz. C. excavata, C. heptaphylla and C. lansium in Assam. The results revealed that the leaves are elliptic in shape and arranged in an imparripinnate manner. In C. heptaphylla and C. excavata, flowers are tetramerous while in C. lansium, the flowers are pentamerous. Star shaped flower bud is one of the distinguishing characters of C. lansium. Stomata are of mainly anomocytic type in all the three selected species whereas in C. heptaphylla and C. lansium, anisocytic type of stomata also found. The pollens are sub-prolate in shape and minutae in size. This study uncovered detailed description on macro and micromorphology and obtained data might be useful as a source of identification and describing the taxonomic status of the genus.
References
Albert S, Sharma B (2013) Comparative foliar micromorphological studies of some Bauhinia (Leguminosae) species. Turk J Bot 37(2):276–281. https://doi.org/10.3906/bot-1201-37
Aleykutty KM, Inamdar JA (1980) Structure, ontogeny and classification of trichomes in Ranales. Feddes Repert 19(1–2):95–108
Babalola KA, Victoria AA (2009) Foliar Epidermal Morphology of Two West African Genera of Haloragaceae R. BR. (Saxifragales). J Sci Res Dev 11:84–91
Barkatullah MI, Jelani G, Ahmad I (2014) Leaf, stem bark and fruit anatomy of Zanthoxylum Armatum dc. (Rutaceae). Pak J Bot 46(4):1343–1349
Bersier JD, Bocquet G (1960) Les methods d’ eclaircis sementen vascularization et en morphologene vegetable compares. Arch Sci (Geneva) 13:555–556
Boulos ST, Beakbane (1971) A chemical method for separating leaf epidermis from mesophyll tissue. J Bot United Arab Repub 14:317–322
Deka K, Kalita N, Das S et al (2024) Habitat mapping through improving the conservation status of genus Clausina- an important medicinal plant of Assam, India. Ecol Environ Conserv 30:S48–S55
Dilcher DL (1974) Approaches to identification of angiosperm leaf remains. Bot Rev 40:1–157
Erdtman G (1952) Pollen morphology and plant taxonomy Angiosperms (An introduction to palynology). Almgvist and Wiksell, Chronica Botanica, Stockholm.
Fan GJ, Han BH, Kang YH, Park MK (2001) Evaluation of inhibitory potentials of Chinese medicinal plants on platelet-activating factor (PAF) receptor binding. Nat Prod Sci 7(2):33–37
Franco C (1939) Relation between chromosome number and stomata in Coffea. Bot Gaz 100 (4): 817–827. https://www.jstor.org/stable/2471773
Guntupalli C, Kumar GS, Kumar AS, Tubati T (2012) Evaluation of antioxidant activity of the methanolic leaf extract of Clausena excavata Burm. F. (Rutaceae) using the lipid peroxidation model. Pharmacogn J 4(34):22–25. https://doi.org/10.5530/pj.2012.34.4
Hickey LJ (1973) Classification of the architecture of dicotyledonous leaves. Amer J Bot 60(1):17–33
Hooker JD (1890) The Flora of British India (1). Missouri Botanical Garden, 1896, London: L. Reeve & Co., 5, Henrietta Street, Covent Garden
Jain SK, Rao RR (1977) A hand book of field and herbarium methods. Today and Tomorrow’s Printers and Publishers, New Delhi. pp 157
Kalita B, Devi N (2021) Leaf archetectural studies in Magnolia hodgsonii (Hook. f. & Thompson) H. Keng. Biological Spectrum of Northeast India, EBH Publishers (India). pp. 186–190
Kalita D, Devi N (2015) Observations on the foliar architecture and micro-morphology of Diospyros lanceifolia Roxburgh (Ebnaceae). Pleione 9(2):449–455
Kanjilal UN, Kanjilal PC, Das A (1934) Flora of Assam. Vol 1 (1). Government of Assam, Shillong, India. pp 983–993
Kokwaro JO (1982) Flora of Tropical East Africa: Rutaceae. pp 52.
Kumar R, Saha A, Saha D (2012) A new antifungal coumarin from Clausena excavata. Fitoterapia 83(1):230–233. https://doi.org/10.1016/j.fitote.2011.11.003
Larano A, Buot IE (2010) Leaf architecture of selected species of Malvaceae sensu APG and its taxonomic significance. Philipp J of Syst Biol 4:21–51
Lawal IO, Uzokwe VNE, Rafiu B, Afolayan AJ (2018) Foliar anatomy of Clausena anisata (Willd.) Hook: a South African medicinal plant. Niger J Nat Prod Med 22:149–154
Liu GT, Li WX, Chen YY, Wei HL (1996) Hepatoprotective action of nine constituents isolated from the leaves of Clausena lasium in mice. Drug Dev Res 39(2):174–178. https://doi.org/10.1002/(SICI)1098-2299(199610)39:2%3c174::AID-DDR10%3e3.0.CO;2-C
Melville R (1976) The terminology of Leaf Architecture. Taxon 25:549–561. https://doi.org/10.2307/1220108
Mendoca FA (1963) Flora. Zambesiaca 2:1
Metcalfe CR (1961) The anatomical approach to systematic: General introduction with special reference to recent work on monocotyledons. In: Bailey DL (ed) Recent advances in botany. University of Toronto Press, Canada, pp 146–150
Metcalfe CR, Chalk L (1979) Anatomy of Dicotyledons. Systematic Anatomy of Leaf and Stem, with a Brief History of the Subject. Clarendon Press, Oxford, pp 40–41
Mou F and Zhang D (2012) Systematic significance of pollen morphology in Clausena (Rutaceae) from China. J Jpn Bot 87(4): 242–252. https://www.researchgate.net/publication/288086233
Nair PKK (1970) Pollen morphological basis for plant classification. J Palyn 6:111–112
Nair KN & Nayar MP (1997) Rutaceae, In Hajra, PK, Nair, V. J. & Daniel, P. (Eds.) Flora of India Vol-4 Malpighiaceae- Dechapetalaceae. Botanical Survey of India. pp. 259–408
Nath M, Dutta Choudhary M (2009) Ethno- Medico-botanical aspect of Hmar tribe of Cachar district, Assam (part I). IJTK 9(4):760–764
Ng TB, Lam SK, Fong WP (2003) A homodimeric sporamin- type trypsin inhibitor with antiproliferative. HIV reverse transcriptase- inhibitory and anti- fungal activities from wampee (Clausena lansium) seeds. Biol Chem 384(2):289–293. https://doi.org/10.1515/bc.2003.032
Ohikhena FU, Wintola OA, Afolayan AJ (2017) Micromorphological Studies of the Loranthaceae, Phragmanthera capitata (Sprengel) Balle. J Bot 2017(1):5603140. https://doi.org/10.1155/2017/5603140
Paul P, Chowdhury M (2021) Foliar micromorphology as a tool for identification of Indian taxa of Polygonaceae. J Asia-Pac Biodivers 14:569–589. https://doi.org/10.1016/j.japb.2021.05.008
Paul S, Devi N (2013) Floral morphology and foliar anatomy of Blumea lanceoria (Roxburgh) Druce [Asteraceae]. Pleione 7(1):32–38
Payne WW (1978) A glossary of plant hair terminology. Brittonia 30(2):239–255. https://doi.org/10.2307/2806659
Ramayya N, Rajagopal T (1980) Classification of subsidiaries according to interstomatal relationship. Curr Sci 14(17):671–673
Ridley HN (1922) The Flora of the Malay Peninsula: Apetalae. L. Reeve and co., ltd.
Salisbury EJ (1927) On cause and ecological significance of stomata frequency with special reference to Woodland Flora. Philos Trans Royal Soc B Biol Sci 216:1–65. https://doi.org/10.1098/rstb.1928.0001
Sharif NWM, Mustahil NA, Mohd Noor NS (2011) Cytotoxic constituents of Clausena excavata. Afr J Biotechnol 10(72):16337–16341. https://doi.org/10.5897/AJB11.2239
Shunmugam S, Rosli NS, Manickamb S, Mohd Yusoffa NF, Alitheena NB, Namasivayama P (2021) Morphology and Anatomy of Leaf, Stem and Petiole of Luvunga crassifolia Tanaka (Rutaceae). Mal J Fund Appl Sci17(6):818–828
Singh S, Manchawal L, Chauhan MG (2010) Pharmacognostic study of male leaves of Trichosanthes dioica Roxb. with special emphasis on microscopic technique. J Pharmacogn Phytotherapy 2(5):71–75. https://doi.org/10.5897/JPP.9000038
Stace CA (1984) The taxonomic importance of leaf surface. In: Heywood VH, Moore DM (eds) Current concepts in plant taxonomy systematic. Academic Press, London, pp 67–94
Sunthitikawinsakul A, Kongkathip N, Kongkathip B (2003) Coumarins and carbazoles from Clausena excavata exhibited antimycobacterial and antifungal activities. Planta Med 69(2):155–157. https://doi.org/10.1055/s-2003-37716
Talukdar RT, Devi N (2016) Foliar architectural and micro-morphological investigations on Pothos scandens Linnaeus (Araceae): an interesting climbing epiphyte from Kamrup District of Assam. Pleione 10(1):8–13
Author Information
Department of Botany, Gauhati University, Guwahati, India